【题目】以下是与高中生物实验有关的内容,请回答下列问题:
(1)脂肪的鉴定中,洗去浮色时常用50%的酒精溶液,是因为 。
(2)在“观察DNA和RNA在细胞中的分布”实验中,将口腔上皮细胞置入8%的盐酸溶液中水解的主要目的是 ,有利于DNA与染色剂结合。
(3)比较过氧化氢在不同条件下的分解,实验的因变量是 。
(4)在用琼脂块模拟细胞大小与物质运输关系的实验中,物质运输的效率可以用 来衡量。
(5)生物组织细胞中某些成分的鉴定和结构的观察,需要选取适当的实验材料、试剂和仪器,下列材料、仪器(试剂)与成分鉴定和结构观察匹配不正确的有( )(多选)
A.西瓜汁~斐林试剂~检测生物组织中的糖类
B.嫩绿的菠菜叶~层析液~提取光合色素
C.口腔上皮细胞~健那绿染液~观察DNA在细胞中的分布
D.新鲜菠菜叶~高倍显微镜~观察叶绿体
【答案】
(1)苏丹Ⅲ染液或苏丹Ⅳ染液能溶于酒精
(2)使染色质中DNA与蛋白质分离
(3)过氧化氢分解速率(或其他合理答案)
(4)NaOH扩散体积/整个琼脂块的体积
(5)A、B、C
【解析】
(1)在脂肪的鉴定中,洗去浮色时常用体积分数为50%的酒精溶液,因为苏丹Ⅲ染液或苏丹Ⅳ染液能溶于酒精。
(2)在“观察DNA和RNA在细胞中的分布”实验中,将口腔上皮细胞置入8%的盐酸溶液中水解的主要目的是使染色质中的DNA与蛋白质分离,有利于DNA与染色剂结合。
(3)比较过氧化氢在不同条件下的分解,实验的因变量是过氧化氢分解速率。
(4)在用琼脂块模拟细胞大小与物质运输关系的实验中,物质运输的效率可以用NaOH扩散体积/整个琼脂块的体积来衡量。
(5)检测生物组织中的还原糖,不用西瓜汁是因为会产生颜色干扰;提取绿叶中的色素应该用深绿的菠菜叶作为材料,使用丙酮或无水乙醇作为溶剂;观察DNA在细胞中的分布,应用甲基绿作为染色剂,健那绿染液是线粒体的染色剂;观察叶绿体可用新鲜菠菜叶作为材料,并借助高倍显微镜观察。故不正确的是A、B、C。
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