[题目]利用细胞工程方法.以SARS病毒核衣壳蛋白为抗原制备出单克隆抗体.下列相关叙述正确的是( )A．用纯化的核衣壳蛋白反复注射到小鼠体内.产生的血清抗体为单克隆抗体B．体外培养单个效应B细胞可以获得大量针对SARS病毒的单克隆抗体C．将等量效应B细胞和骨髓瘤细胞混合.经PEG诱导融合后的细胞均为杂交瘤细题目和参考答案——青夏教育精英家教网—

【题目】利用细胞工程方法，以SARS病毒核衣壳蛋白为抗原制备出单克隆抗体。下列相关叙述正确的是（）

A．用纯化的核衣壳蛋白反复注射到小鼠体内，产生的血清抗体为单克隆抗体

B．体外培养单个效应B细胞可以获得大量针对SARS病毒的单克隆抗体

C．将等量效应B细胞和骨髓瘤细胞混合，经PEG诱导融合后的细胞均为杂交瘤细胞

D．利用该单克隆抗体与SARS病毒核衣壳蛋白特异性结合的方法可诊断出病毒感染者

【答案】D

【解析】单克隆抗体是效应B细胞与骨髓瘤细胞融合形成的杂交瘤细胞分泌的，A错误；单独的效应B细胞能产生抗体，但不能无限增殖，因此在体外培养的情况下，不能得到大量的单克隆抗体，B错误；将等量效应B细胞和骨髓瘤细胞混合，经PEG诱导融合后的细胞有效应B细胞自身融合细胞、骨髓瘤细胞自身融合细胞和杂交瘤细胞三种，C错误；单克隆抗体具有特异性强、灵敏度高的特点，因此利用该单克隆抗体与SARS病毒核衣壳蛋白特异性结合的方法可诊断出病毒感染者，D正确。

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