Fifteen is such an awful(糟糕的)age, I should know. I really wish I were eighteen. If you are an adult, you can be your own boss. People like my parents and my teachers are always telling me what to do. You know what I mean. "It's time for bed, Sandy." Or "Sandy, you've watched too much TV for one night." "Sandy, stand up straight." "Sandy, you must improve your handwriting." Sandy this, Sandy that. No one tells adults when they go to bed or not to watch TV. Besides, adults get to wear what they like... Now it's going to be great to be an adult. Don't you agree?

I'm Sandy's mother. Having a fifteen-year-old daughter isn't always easy. Right now, we've got a problem. Our neigh hour, Mrs. White, wants Sandy to look after her pet dog for one week. Sandy really wants to do it, but (I         ).  She's too young and inexperienced. Well, I will never be able to relax knowing she is all alone with a dog. I've tried to explain my feelings to her, but she won't listen. She keeps telling me that the Whites live very near, so she can ask for help. She insists that Mrs. White won't ask her to look after her pet dog if she doesn't think Sandy is old enough. She says I'm "babying" her and I hate to see her mad at me. What should I do?

1. Sandy is fifteen years old and she wishes to be her own_______.

2. Sandy thinks her parents and her teachers are always telling her what to do. Give an example.

3．将文中括号内的英文句子补充完整。

4．将划线句子She says I'm "babying" her and I hate to see her mad at me.译成汉语。

5. Should Sandy look after Mrs. White's pet dog? Why or why not?

科学家将外源目的基因与大肠杆菌的质粒进行重组，并在大肠杆菌中成功表达。下图表示构建重组质粒和筛选含目的基因的大肠杆菌的过程。请据图回答：

（1）步骤①和②中常用的工具酶是                            。
（2）经过①和②步骤后，有些质粒上的                基因内插入了外源目的基因，形成重组质粒。
（3）步骤③是                                 的过程。
（4）步骤④：将三角瓶内的大肠杆菌接种到含四环素的培养基C上培养，目的是筛选     。
（5）步骤⑤:用无菌牙签挑取C上的单个菌落，分别接种到D（含氨苄青霉素和四环素）和E（含四环素）两个培养基的相同位置上，一段时间后，菌落的生长状况如图所示。含目的基因的菌落位于（选填“D”或“E”）      上。