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4.    by the Revolution,France became a different country afterwards,and today, it is still  guided by those      .

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3.Many people died of    . Ten years later, the    of the French Revolution led to  fundamental changes throughout the country.

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2.The Temple of Nike is the smallest among the three.It used to    a 13 – metre – high – gold – covered     of the goddess of Victory.

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1.For a period of about 300 years (from 650 to 323 BC), the Greek civilization made     in various fields that have     the world for more than 2,500 years already, and will continue to do so.

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第二节(共15小题)

请听第6段材料,回答第6、7题。  

6.What will the two speakers do tomorrow morning?

     A.Invite Mr. Green to dinner.  

    B.Visit Mr. Green’s new house.

     C.Go to see their parents.

7.What will the man do tomorrow afternoon?

     A.Do some writing.                        B.Go swimming.    C.Go fishing

请听第7段材料,回答第8至10题。

8.What does the man want to drink?

     A.Water.           B.Tea.              C.Coffee.

9.What is the man doing?

    A.Watching TV       B.Writing some reports.    C.Listening to the radio.

10.When does this conversation take place?

     A.Late at night.     B.In the afternoon.   C.In the morning.

请听第8段材料,回答第11至14题。

11.What is the relationship between the two speakers?

     A.Manager and secretary.                   B.Husband and wife. C.Neighbors.

12.Why did the man’s classmates come to Guangzhou?

    A.To attend a conference.                 B.To visit the city.   C.To see the man.

13.What did the man ask his classmates to do at first?

    A.He invited them to dinner in a hotel.  

    B.He asked them to go shopping with him.

    C.He invited them to have a chat in his office.

14.What does the woman suggest?

    A.She suggests buying some girls for the two classmates.

    B.She suggests showing the two classmates around the whole city.

    C.She suggests treating the two classmates at home.

请听第9段材料,回答第15至17题。

15.Why did the woman go to Japan?

    A.To study.           B.To visit her friends.    C.To travel.

16.Why didn’t the woman see many places in Japan?

    A.She didn’t want to.   B.The weather was bad.   C.She was not free.

17.How long did the woman stay in Japan?

    A.For about three months.                   B.For about two months.   C.For about one month.

请听第10段材料,回答第18至20题。

18.What did football players wear before the 20th century?

    A.They wore long trousers and hats.

    B.They wore shorts and vests.

    C.They wore black trousers and jackets.

19.When did referees first appear in football matches?

    A.In 1872.            B.In 1891.            C.In the 20th century.

20.How did the idea of red and yellow cards come about?

    A.It came from traffic lights.                 

    B.It carne from street lights.

    C.It came from lights on the cars.

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第一节(共5小题)

1.What does the woman ask the man to do?

     A.Take her to the Smith Hotel.  

    B.Show her the way to the Smith Hotel.

     C.Show her the way to the hospital.

2.What does the woman want to do?

     A.Open the door.     B.Let the man in.     C.Open the window.

3.What will the woman help the man to do?

     A.Carry some books.                       B.Pick up some books.    C.Buy some books.

4.What do the man’s parents usually do in the evening?

     A.Do some shopping.                      B.Watch TV.     C.Do a lot of reading.

5.Why couldn’t the woman get through to the man? 

     A.His mobile was stolen.  

    B.His mobile didn’t work.

     C.His mobile was power off.

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43.(8分)萌发的禾谷类种子中淀粉酶活性较强,主要有α-淀粉酶和β-淀粉酶。α-淀粉酶不耐酸、较耐热,在PH为3.6以下迅速失活,而β-淀粉酶不耐热,在70℃ 15min后失活。根据它们的这种特性,可分别测定一种酶的催化效率。请协助完成“测定小麦种子中α-淀粉酶催化效率”的实验。

    实验材料:萌发3天的小麦种子(芽长约1cm)。

    主要试剂及仪器:1mg/mL的标准麦芽糖溶液、5%淀粉溶液、斐林试剂、蒸馏水、石英砂恒温水浴锅等。

    实验步骤:

    步骤一:制作麦芽糖标准。取7支干净的具塞刻度试管,编号,按表加入试剂,再将试管置于60℃水浴中加热2min,取出后按试管号顺序排列。

试剂
试管号
1
2
3
4
5
6
7
麦芽糖标准液(mL)
0
0.2
0.6
1.0
1.4
1.6
2.0
蒸馏水(mL)
2.0






麦芽糖含量(mg)
0
0.2
0.6
1.0
1.4
1.6
2.0
斐林试剂(mL)
2.0
2.0
2.0
2.0
2.0
2.0
2.0

步骤二:制备酶液。

步骤三:将装有淀粉酶溶液的试管置于         ,取出后迅速冷却。

    步骤四:另取四支试管,编号A、B、C、D,向A、B试管中各加5mL5%淀粉溶液,向C、D试管中分别加入2mL 已经处理的酶溶液(忽略少量的麦芽糖)和蒸馏水,将四支试管置于40℃恒温水浴中保温10min,然后将C、D试管中的溶液分别加入到A、B试管中,摇匀后继续在40℃恒温水浴中保温l0min。

    步骤五:              

    结果分析:将A试管中颜色与第①步中获得的麦芽糖标准进行比较,获得该试管中麦芽糖浓度,并计算出α-淀粉酶催化效率。

    分析上述步骤回答:

  (1)步骤一的2-7试管中加入蒸馏水的量分别是        (单位mL)。

  (2)步骤三的实验操作是                           

  (3)步骤五的实验操作是                           

  (4)实验中B试管所起的具体作用是                       

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42.(6分)为了解基因结构,通常选取一特定长度的线性DNA分子,先用一种限制酶切割,通过电泳技术将单酶水解片段分离,计算相对大小;然后再用另一种酶对单酶水解片段进行讲解,分析片段大小。下表是某小组进行的相关实验。

 
 
已知第一线性
DNA序列共有
5000bp
(bp为碱基对)
第一步水解
产物
(单位bp)
第二步水解
产物
(单位bp)
 
A酶切割
3500
将第一步水解产物分离后,分别用B酶切割
1500  2000
1000
1000
500
500
B酶切割
2000
将第一步水解产物分离后,分别用A酶切割
500  1500
3000
2000  1000
经A酶和B酶同时切割
2000  1500  1000  500

  (1)该实验中体现出限制酶的作用特点是               

  (2)由实验可知,在这段已知序列上,A酶与B酶的识别序列分别为       个和        个。

  (3)根据表中数据,请在下图中用箭头标出相应限制酶的酶切位点。

  (4)已知BamH I与BglⅡ的识别序列及切割位

点如右图所示,用这两种酶和DNA连接酶

对一段含有数个BamH I和BglⅡ识别序列

的DNA分子进行反复的切割、连接操作,

若干次循环后      和       序列明显增多,该过程中DNA连接酶催

    键的形成。

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41.(7分)果蝇的长翅(A)对残翅(a)是显性,红眼(B)对白眼(b)是显性。左下图为某果蝇的染色体及基因组成,右下表为该果蝇与“另一亲本”杂交后代的表现型

 
长翅红眼
长翅白眼
残翅红眼
残翅白眼
雌蝇



0
雄蝇




  (1)分析上图可知,该果蝇的性别为     ,其一个正常的体细胞包括    个染色体组。“另一亲本”果蝇的基因型为       

  (2)若上图果蝇一个原始生殖细胞产生的一个配子的基因型为AAY,则同时产生的另三个子细胞的基因组成分别为       

  (3)该果蝇与“另一亲本”杂交,子代的长翅红眼雌果蝇中杂合体占的比例为    

  (4)若只根据子代果蝇的眼色就能判断其性别,则选作亲本果蝇的眼色基因型应该为

             

  (5)若选择长翅红眼雌果蝇和长翅白眼雄果蝇两种亲本杂交,子代雌雄果蝇都有上表所示四种表现型,且比例为3:3:1:1,则选作亲本果蝇的基因型应该为    

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40.(6分)植物的光合作用受多种内、外因素的影响。在相同条件下,不同植物光合速率不同是由植物本身的遗传特性决定的;同一品种植物的光合速率主要受光照、CO2浓度、温度、水分、矿质营养等环境因素的影响。甲图是在乙图m点的条件下测得的曲线,乙图是在甲图c点的条件下测得的曲线,请依图回答下列问题:

  (1)限制甲图C点后光合作用速率增加的主要环境因素是         。(至少2点)。

  (2)甲图两曲线的差异是由植物本身的       决定的。

  (3)乙图中两曲线后段呈下降趋势的原因是      

  (4)如在乙图的n点条件下测得阳生植物光照强度与光合CO2同化速率关系曲线,则该曲线与甲图的阳生植物曲线比较b、c点的移动情况是      

  (5)试提出农业生产中充分利用光能的一种措施:      

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