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第一节  单词拼写(共5小题,每小题1分,满分为5分)

根据句意完成句子,在空格里写出恰当形式的英语单词,每空一词,将答案填在答题卡上。

61.  If you do the same thing over and over again, you begin to do it _____________(automatic).

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第二节:听取信息(共5小题;每小题1分,满分5分)

   听取下面一段独白,请根据题目要求,从所听到的内容中获取必要的信息,然后填入标号为16-20的空格中。

 
 
Some advice on cycling tours
Safety: to wear something that can easily be (16)________
(17)__________and speed: not to ride too far or too (18)_________
Drink: to take plenty of water - at least (19)________liter every hour.
In cold weather: to take some warm clothes and put them on when you
stop(20)_________

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第一节:听力理解(共15小题;每小题1分,满分15分)

请听下面5段对话或独白,选出最佳选项。

请听第1段对话,回答第1至3题。

1. What did the man do last night?

A. He drank at a restaurant.      B. He saw a film.      C. He went to Alice’ house.

2. What does the woman suggest the man do at last?

  A. Go to cinema early.          B. Buy a ticket for her.  C. Take some tissues with him.

3. What can we know from the conversation?

  A. The man doesn’t like drinking. 

B. The woman lost her cell phone last night.

  C. The woman will probably see a film this evening.

请听第2段对话,回答第4至6题。

4. What’s the matter with the man’s father?

  A. He can’t look after himself.    B. His left hand can’t be moved  

C. He feels very lonely.

5. What can make the man’s father happy?

  A. Money.                 B. Mike’s visit.      C. Convenient life.

6. Why doesn’t the man’s father come to live with him?

  A. He doesn’t want to bother his son.

  B. He can’t often talk with his friends and the villagers.

  C. It isn’t convenient to live here.

请听第3段对话,回答第7至9题。

7. What does the man mainly do on the Internet?

A. He checks his e-mails.         B. He finds products and components.

C. He surfs the net.

8. What’s the man’s opinion about the Internet?

A. Online information is very new.  B. We shouldn’t buy anything online.

C. A lot of information online is false.

9. How does the man buy something online?

A. Checking the websites of shops carefully.

B. Sending someone to the shops to check the products.

C. Going to the shop by himself.

请听第4段独白,回答第10至12题。

10. What is the speaker mainly talking about?

  A. A historic tree.             B. A well-known park.   C. A famous commander.

11. What do we know about the Washington Elm?

  A. It was less than one hundred years old.      

B. Washington couldn’t have commanded under it.     

C. It was much older than people had thought.

12. What can we learn from the talk?

  A. Some historical stories are untrue.

  B. Washington commanded the army on his knees.     

C. The Washington Elm was destroyed before the celebration.

请听第5段对话,回答第13至15题。

13. Why does the man find the Olympics interesting?

  A. He thinks the Olympics need a lot of money.   

B. He thinks the Olympics can cause many wars in the world.   

C. He thinks many people just want to show their countries are stronger. 

14. What’s the woman’s opinion about the Olympics?

  A. The Olympics help to promote world peace.    

B. There’s no real spirit of cooperation in the Olympics.   

C. Many athletes are not friendly to each other.

15. What are the two speakers in agreement with?

  A. The Olympics for the disabled are very amazing.

  B. Taking part in the Olympics is uninteresting.

  C. Most people lack true speed, skill, ability and strength.

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20.(20分)现有甲乙两种可能有一定毒性的化学物质,实验室研究人员欲通过动物细胞培养的方法来鉴定它们是否有毒性并比较二者毒性的强弱。请完成下列实验报告并回答有关问题。

Ⅰ 实验原理:有毒物质加入细胞培养液后,培养的动物细胞会发生染色体变异。根据这种变异细胞占全部培养细胞的百分数(以下称Q值),可判断该化学物质的毒性。

Ⅱ 实验材料:活小白鼠胚胎。

Ⅳ 实验步骤:

(1)制备动物细胞培养液:

①严格按照动物细胞所需营养物质的           配制合成培养基。

②在合成培养基中加入适量        ,以补充合成培养基中所缺乏的营养物质。

(2)制备细胞悬浮液:把活小白鼠胚胎放在培养皿中剪碎,加入        ,使胚胎组织离散成单个细胞,再加入动物细胞培养液,制成细胞悬浮液。

(3)进行细胞培养:

①取A、B、C三个洁净的培养瓶,分别加入            

②向A培养瓶中加入化学物质甲,向B培养瓶中加入          ,并将培养瓶摇匀。

③把三个培养瓶放在温度适宜、含5%CO2的恒温箱中培养。此处CO2的作用是      

(4)制作临时装片:

①培养一段时间后,同时从恒温箱中取出三个培养瓶,用      处理,使培养的细胞从瓶壁上脱落。

②吸取适量的细胞,制作成临时装片,用显微镜观测。

③统计A、B、C三个培养瓶中变异的细胞的Q值,分别记作QA、QB、QC

Ⅴ 实验结果和结论:

(5)若实际测得QA=0.22%、QB=13.1%、QC=0.21%,说明                 

(6)若实际测得QA=1.3%、QB=12.5%、QC=0.1%,说明                  

Ⅵ 回答问题:

(7)在实验中,C培养瓶起到了         作用。

湛江一中2009-2010年度第一学期高二级12月质量检测

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19.(14分)现有甲、乙两个蕃茄品种,其基因型分别为yyRr和Yyrr。科学家将它们的花粉除去细胞壁后进行原生质体融合,再把这些融合细胞进行组织培养,培育出了蕃茄新品种。操作过程如下:

(1)上述育种方法采用的生物技术称作            技术,这项技术的重要意义是能够克服           的障碍。

(2)两个不同的原生质体能够相互融合,其生物学原理是         ,融合时常用的化学诱导剂是           

(3)若需要制备该新品种蕃茄的人工种子,可在d步骤选取具有生根发芽能力的    (结构),包裹上人造种皮制得。

(4)两个品种的花粉进行原生质体融合后(仅考虑两两融合),可产生       种基因型的融合细胞,这些融合细胞再经过组织培养可以得到        种不同于亲本表现型的玉米新品种。

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