25. How I wish I _____ my mouth before I shouted at my mum!
A. shut B. have shut C. had shut D. would shut
24. The cruel parents ____ because of ____ they had done to the little girl.
A. got punishment; which B. got punished; what
C. got punishing; that D. got to be punished; why
23. I was so familiar with him that I recognized his voice ____ I pick up the phone.
A. while B. after C. in case D. the moment
22. Mom’s spent the day shopping, cleaning and cooking and now she_____.
A. has worn out B. is worn out C. is wearing out D. wears out
第一节:单词填空(共15小题;每小题1分,满分15分)
21. The photo on _____ cover of the book may cause_____offence to some people.
A. the; 不填 B. the; an C. the; the D. 不填; the
第二节(共15小题;每小题1. 5分,满分22. 5分)
请听下面5段对话或独白,选出最佳选项。
请听第6段材料,回答第6至8题。
6. What’s the time now?
A. 9:54 B. 10:00 C. 11:54
7. How soon should the ambulance and the police car arrive there?
A. In at most 4minutes. B. In at most 6 minutes. C. In more than 10minutes.
8. What can we know about the accident?
A. A passenger was injured.
B. The taxi was damaged.
C. The truck driver was hurt.
请听第7 段材料,回答第9至11题。
9. What is the man probably doing?
A. Drawing a picture. B. Painting the house. C. Clearing the house.
10. What will the woman do to help the man?
A. Buy some paint. B. Clean the carpet. C. Make some curtains.
11. Why does the man say sorry to the woman?
A. He made a mess in the house.
B. He dropped some paint on the carpet.
C. He used a color the woman doesn’t like.
请听第8段材料,回答第12至14题。
12. Where was the woman when the hurricane struck?
A. In her father’s friend’s house.
B. In her own house with her husband.
C. On her way home.
13. When did the hurricane hit the area?
A. At about 3:30 am on August 29.
B. At about 5:00 am in August 30.
C. At noon on August 30.
14. Why did the woman have to drive slowly?
A. Too many cars were on the road.
B. There was a very strong wind.
C. Broken tree branches were everywhere.
请听第9段材料,回答第15至17小题。
15. Where does the conversation probably take place?
A. In a cinema. B. In a restaurant. C. At an office.
16. What have the speakers just seen?
A. A game. B. A talk show. C. A movie.
17. What’s the man worried about?
A. Whether he’ll be given the job.
B. His coming interview.
C. Whether he can get the film tickets.
请听第9段材料,回答第18至20题。
18. Who is the speaker probably?
A. A student. B. A basketball player. C. A manager.
19. What time does the center open?
A. At 9:00 am. B. At 2:00 pm. C. At 5:00 pm.
20. What is the speaker mainly talking about?
A. Different sports rules.
B. Different indoor sports.
C. Different sporting activities.
第一节(共5小题; 每小题1. 5分,满分7. 5分)
请听下面五段对话,选出最佳选项。
1. What will the woman probably do tonight?
A. See a film. B. Attend a meeting C. Stay at home
2. What do we know about Susan?
A. She likes to go hiking.
B. She is a travel agent.
C. She is very humorous.
3. Where are the two speakers probably?
A. On a farm. B. At a post office. C. In a store.
4. What does the woman mean?
A. She doesn’t want to see the professor at all.
B. She hopes to see the professor after the others do.
C. She wants the professor to work on the project with her.
5. What time will the speakers get to Beijing?
A. At11:00 B. At 12:30 C. At 12:45
52.现有甲、乙两个烟草品种(2n=48),其基因型分别为aaBB和AAbb,这两对基因位于非同源染色体上,且在光照强度大于800勒克司时,都不能生长,这是由于它们中的一对隐性纯合基因(aa或bb)作用的结果。
取甲乙两品种的花粉分别培养成植株,将它们的叶肉细胞制成原生质体,并将两者相混,使之融合,诱导产生细胞团。然后,放到大于800勒克司光照下培养,结果有的细胞团不能分化,有的能分化发育成植株。请回答下列问题:
(1)甲烟草品种花粉的基因型为 ;
(2)将叶肉细胞制成原生质体时,使用 破除细胞壁;
(3)在细胞融合技术中,常用的促融剂是 ;
(4)细胞融合后诱导产生的细胞团叫 ;
(5)在大于800勒克司光照下培养,有 种细胞团不能分化,能分化的细胞团是由 的原生质体融合来的(这里只考虑2个原生质体的相互融合)。由该细胞团分化发育成的植株,其染色体数是 ,基因型是 。该植株自交后代中,在大于800勒克司光照下,出现不能生长的植株的概率是 。
53.下面甲图中DNA决定某一多肽链中的酪氨酸和丙氨酸过程示意图,乙图示样品 DNA经PCR技术(聚合酶链式反应)扩增,可以获取大量DNA克隆分子。分析回答:
(1)甲图中含有 种核苷酸;丙氨酸的遗传密码子是 。该图表示了DNA中遗传信息的 过程。
(2)有一种贫血症是血红蛋白分子的一条多肽链上,一个酪氨酸被一个苯丙氨酸所替代造成的。此种贫血症的根本原因是 ,即 发生了改变。
(3)乙图的“PCR”与人体内的DNA复制相比有何特殊之处? 。
(4)现在要通过“PCR”得到甲图中DNA片段的1024个克隆片段,则至少要向试管中加入个腺嘌呤脱氧核苷酸。
51.转基因抗病香蕉的培育过程如图所示。质粒上有PstⅠ、SmaⅠ、EcoRⅠ、ApaⅠ等四种限制酶切割位点。请回答:
(1)构建含抗病基因的表达载体A时,应选用限制酶 和 ,
对 和 进行切割;
(2)培养基中的卡那霉素会抑制香蕉愈伤组织细胞的生长,欲利用该培养筛选已导入抗病基因的香蕉细胞,应使基因表达载体A中含有 ,作为标记基因;
(3)香蕉组织细胞具有 ,因此,可以利用组织培养技术将导入抗病基
因的香蕉组织细胞培育成植株。图中①、②依次表示组织培养过程中香蕉组织细胞的 和 。
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