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25. A. accepted      B. agreed          C. apologized       D. refused

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24. A. when        B. if only          C. after           D. unless

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23. A. discussion    B. agreement       C. disagreement     D. fight

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22. A. make        B. correct          C. keep           D. decide

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第一节  听力理解(5段共15小题;每小题2分,满分30分)

每段播放两遍。各段后有几个小题,各段播放前每小题有5秒钟的阅题时间。请根据各段播放内容及其相关小题,在5秒钟内从题中所给的A、B、C项中,选出最佳选项,并在答题卡上将该项涂黑。

听第一段对话,回答第1-3题。

1. What is the man’s orginal opiniopn of the Ice Age people?

A. They were surprisingly advanced.

B. They were primary cave dwellers.

C. They were inventors of art and music.

2. How did the early Ice Age people keep warm?

A. They put heated stones on the roof.

B. They faced their houses towards the south.

C. They covered their houses with animal skins.

3. Why does the man want to read the article about the Ice Age people?

A. To impress his teacher.

B. To write his homework.

C. To fulfill his curiousity.

听第二段独白,回答第4-6题。

4. What does the speaker think the biggest problem is?

A. Packaging.

B. People spitting.

C. People not cleaning up after their dogs.

5. Why is spitting on the street bad?

A. Because it is dirty and can spread illnesses.

B. Because it is dirty and makes the street look bad.

C. Because it is not nice for others to see someone spitting.

6. What can you help do in your area if you walk or ride a bike more often?

A. Reduce using gases.

B. Cut down the pollution.

C. Improve the traffic.

听第三段对话,回答第7-9题。

7. Where did the woman find the information about the job?

A. From an advertisement.

B. From her teacher.

C. In a newspaper.

8. When is the woman expected to work Monday through Friday?

A. 4:00 p. m. to 6:00 p. m.

B. 4:30 p. m. to 6:30 p. m.

C. 6:00 p. m. to 10:00 p. m.

9. What can we learn from the conversation?

A. The woman studied Spanish in high school.

B. The cassettes in the yellow boxes are French lessons.

C. The man will contact the woman in two weeks.

听第四段对话,回答第10-12题。

10. What are the two speakers talking about?

A. The National Day holiday.

B. A vacation plan.

C. A reservation in some cities.

11. How does the woman feel about Xi’an?

A. Beautiful but not big enough.

B. Worth going but too far away.

C. Beautiful but there are too many people.

12. What advice does the woman give in the end?

A. Going to a hot spring resort.

B. Reserving a room as soon as possible.

C. Going to a big city like Shanghai.

听第五段独白,回答第13-15题。

13. What should teens learn according to the talk?

A. How to manage some financial matters well.

B. How to be a financial manager.

C. How to help to support the family.

14. What does it mean by Warren Buffett’s statement?

A. The young generation do not know what they are doing.

B. If people do not understand how to look after their money, there will be economic crisis.

C. The new generation do not know they are taking risks.

15. What is the talk mainly about?

A. The current economic situation has a negative influence on America.

B. Teens should learn to handle money matters on their own.

C. Teens should be taught to be financially independent.

第二节  听取信息(共5小题;每小题1分,满分5分)

听下面一段对话,请根据题目要求,从所听到的内容中获取必要的信息,填入答题卡标号为16-20的空格中。听录音前,你将有10秒钟的阅题时间。录音读两遍,你将有60秒钟的作答时间。

Letters
The mail
Probably a lot of   16
The return name and address of the personal letter
Pete Waters,    17   , Macon County, Georgia
The relationship between Pete Waters and Grandpa
18    in college
The purpose of the letter
To invite Grandpa to attend a    19    for making a 50-year    20

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4.某实验小组为了探究细胞膜的通透性,将小鼠肝细胞在体外培养一段时间后,检测培养液中的氨基酸、葡萄糖和尿素含量,发现它们的含量发生了明显得变化(如下图)。请回答问题。

(1)由图可知,随培养时间延长,培养液中葡萄糖和氨基酸含量     ,尿素含量       。由于在原培养液中没有尿素,推测其是      的产物。

(2)培养液中的氨基酸进入细胞后,其主要作用是      ;被吸收的葡萄糖主要通过

      作用,为细胞提供    

(3)转氨酶是肝细胞内参与氨基酸分解与合成的一类酶,正常情况下这类酶不会排出胞外,若在细胞培养液中检测到该类酶,可能的原因是    

(4)由(1)和(3)可初步判断,细胞膜对物质的转运具有      的特性。

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3、下图为胚胎移植的基本程序、早期胚胎发育过程(图甲),及经过体外受精和胚胎分割移植培育优质奶牛的过程(图乙).请回答下列问题:

⑴ 超数排卵技术的处理措施是对供体母牛注射    。冲卵实质上是用特定装置,将供体母牛子宫内的    冲洗出来。

⑵图乙中②指的是    。进行胚胎分割时,应选择发育到    时期的胚进行操作。

⑶ 图甲中标号3为    ,它将来可发育为     。

⑷ 若一次给受体母牛移入多个胚胎,产生的多个后代基因型相同吗?    ,为什么?   

通过胚胎分割产生的两个或多个个体共有的根本特点是   

⑸ 图乙中A细胞是    ,在进行体外受精前要对精子进行    处理。

哺乳动物受精过程有哪些机制阻止多精子入卵受精?(    )

A.顶体反应      B.精子穿越卵子透明带触及卵黄膜,导致透明带反应

C.精子触发卵黄膜表面微绒毛的抱合反应   D.精子入卵后,触发卵黄膜封闭作用

⑹ 在对囊胚阶段的胚胎进行分割时,应注意   

⑺ 受体母牛必须和供体牛属于    。移植后的胚胎能在受体子宫中存活的生理基础是   

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2、下列是制备分泌抗原X抗体的过程,根据图解回答问题:

⑴ 制备单克隆抗体的步骤:

① 将   注入小鼠体内,从小鼠脾脏中获得淋巴细胞,其中至少1个淋巴细胞带有抗该抗原的抗体。

② 将步骤①获得的B淋巴细胞与步骤②培养的骨髓瘤细胞诱导融合。

③ 将诱导后产生的多种细胞,放在含  培养基的多孔培养板上培养,筛选。

④ 这样获得的杂交瘤细胞具有B淋巴细胞   的能力。

⑤ 检测和分离带有抗X抗体的阳性细 胞,培养板中每孔放一个细胞进行培养。

⑥ 将阳性细胞进行增殖,从而制备出大量抗该抗原的单克隆抗体。

⑵ 单克隆抗体是由同样的抗体分子组成,与一般的血清抗体相比,单克隆抗体的优点是  

⑶ 用本法制备出的单克隆抗体仍具有局限性,因为它是用小鼠的   细胞制备的,其化学本质是   ,对人类有异物性和免疫原性。

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1.下图是某种动物细胞生活周期中染色体变化图,纵坐标表示染色体变化的数目(条),横坐标表示时间。请回答:

(1)图中细胞分裂的次数为______次,___________阶段表示一个有丝分裂的细胞周期。

(2)图中B-C染色体数目发生变化是由于____________分离,F-G染色体数目发生变化是由于____________分离,G-H过程中染色单体数目为____________条。

(3)K-L时产生的细胞是____________,L-M表示________作用,染色体数目加倍。

(4)M-N有____________对同源染色体,O-P有____________条染色单体,图中U时产生的细胞是____________。

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