27. Be careful. Don’t turn the room _______, as I have just tidied it up.
A. upside down B. inside C. outside D. inside down
26. --- Does the idea of staying over night to watch the 2010 World Cup ______ to you?
--- Well, it depends.
A. attract B. suit C. appeal D. match
25. We ______ such great success without your efforts. Thanks a lot.
A. shouldn’t have accomplished B. couldn’t have accomplished
C. were unable to accomplished D. mustn’t have accomplished
24. The Expo park should be ______ from anywhere in the city of Shanghai within 90 minutes by bus, subways and special taxis.
A. adoptable B. tangible C. available D. accessible
23. The possibility of a way out of the difficulty never _____ to the captain and his crew.
A. happened B. occurred C. appeared D. flashed
22. --- What do you think about Chinese knot in the shop window?
--- Oh, it’s so beautiful. You may give it to Debbie as gift for the Dragon Boat Festival.
A. a; a B. the; a C. a; the D. the; the
从A、B、C、D 四个选项中, 选出可以填入空白处的最佳选项,并用铅笔把答题卡上对应题目的答案标号涂黑。
第一节 单项填空(共15小题;每小题1分,满分15分)
21. --- How would you like your Cappuccino coffee?
--- ______.
A. It’s well done B. The stronger, the better.
C. One cup. That’s enough D. Very nice. Thank you.
45.(10分)酵母菌的维生素、蛋白质含量高,可生产食品和药品。科学家将大麦细胞的LTP1基因植入啤酒酵母菌中,获得的啤酒酵母菌种可产LTP1蛋白,并酿出泡沫丰富的啤酒。基本的操作过程如下:
(1)本操作为了将LTP1基因导入啤酒酵母细胞内,所用的运载体是 ,其所具备的两个条件是 、 。
(2)此操作中可以用分别含有X毒素、Y毒素的两种选择培养基进行筛
选,则转基因酵母菌在选择培养基上的生长情况是
;
(3)除了看啤酒泡沫丰富与否外,还可以怎样检测LTP1基因在啤酒酵母中的表达? 。
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