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27. Be careful. Don’t turn the room _______, as I have just tidied it up.

A. upside down    B. inside       C. outside      D. inside down

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26. --- Does the idea of staying over night to watch the 2010 World Cup ______ to you?

  --- Well, it depends.

  A. attract      B. suit        C. appeal       D. match

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25. We ______ such great success without your efforts. Thanks a lot.

  A. shouldn’t have accomplished         B. couldn’t have accomplished

C. were unable to accomplished          D. mustn’t have accomplished

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24. The Expo park should be ______ from anywhere in the city of Shanghai within 90 minutes by bus, subways and special taxis.

A. adoptable    B. tangible      C. available          D. accessible

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23. The possibility of a way out of the difficulty never _____ to the captain and his crew.

A. happened     B. occurred      C. appeared     D. flashed

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22. --- What do you think about     Chinese knot in the shop window?

  --- Oh, it’s so beautiful. You may give it to Debbie as     gift for the Dragon Boat Festival.

A. a; a         B. the; a        C. a; the         D. the; the

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   从A、B、C、D 四个选项中, 选出可以填入空白处的最佳选项,并用铅笔把答题卡上对应题目的答案标号涂黑。

第一节 单项填空(共15小题;每小题1分,满分15分)

21. --- How would you like your Cappuccino coffee?

--- ______.

A. It’s well done               B. The stronger, the better.

C. One cup. That’s enough         D. Very nice. Thank you.

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45.(10分)酵母菌的维生素、蛋白质含量高,可生产食品和药品。科学家将大麦细胞的LTP1基因植入啤酒酵母菌中,获得的啤酒酵母菌种可产LTP1蛋白,并酿出泡沫丰富的啤酒。基本的操作过程如下:

(1)本操作为了将LTP1基因导入啤酒酵母细胞内,所用的运载体是   ,其所具备的两个条件是                     

(2)此操作中可以用分别含有X毒素、Y毒素的两种选择培养基进行筛

选,则转基因酵母菌在选择培养基上的生长情况是         

                               

(3)除了看啤酒泡沫丰富与否外,还可以怎样检测LTP1基因在啤酒酵母中的表达?                      

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