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第一节  单项填空(共15小题,每小题1分, 满分15分)

从每题所给的A、B、C、D四个选项中,选出可以填入空白处的最佳选项

21.She defended she was______ and_____ she would never betray her country.

A. innocent, what    B. pure, that    C. innocent, that    D. innocent, /

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第三节 填空(共三小题,每小题1.5分,满分4.5分)

 听下面一段材料,从听到的内容中获取必要信息,将18-20小题补充完整,每空不超过3个单词,本段材料读两遍。

For 99% of human history
People took food from the world around them.
In the south of China
People eat rice.
In the north of China
People eat 18__________.
In Germany and 19_______
There are many different kinds of sausages.
In north America, Australia
People eat with 20______________.

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第二节(共12小题,每小题1.5分,满分18分)

听下面4段对话,每段对话后有几个小题,从题中所给的A、B、C三个选项中选出最佳选项,并标在试卷的相应位置。每段对话读两遍。

听下面一段材料,回答第6-8题。

6. What is the woman planning to do this evening?

  A. Have a talk with Bob.               B. See some Americans off.

  C. Have dinner with some American friends.

7. What’s wrong with the man?

  A. He’s got a headache.               B. He’s forgotten the date. 

C. He’s forgotten to take some medicine.

8. Who is the man going to write to?

  A. His friends.         B. His parents.     C. His brother.

听下面一段材料,回答第9-11题。

9. What does the man ask the woman to do on Sunday afternoon?

  A. Meet his friends.    B. Watch a volleyball game.  C. Come to a dinner party.

10. What will the woman do on Sunday afternoon?

  A. Invite some friends.   B. Go to Mexico City.    C. Pick up her brother-in-law.

11. What does the man advise the woman to do?

A.    Come over after getting back from the airport.

B.    Bring something to eat.             C. Order pizza for dinner.

听下面一段材料,回答第12-14题。

12. How did the woman know about the man’s wonderful performances?

  A. By watching TV.    B. From the radio.      C. By reading the newspaper.

13. What can we learn about the man?

A. He has traveled a lot.   B. He’s ill.         C. He has a lot of money.

14. What is the man planning to do?

  A. Play with disabled children.  B. Run a special school.  C. Improve his playing skills.

听下面一段材料,回答第15-17题。

15. When does the conversation probably take place?

  A. In the morning.      B. At noon.        C. In the afternoon.

16. Where did the woman go first?

  A. The police station.     B. A restaurant.       C. A shop.

17. Who does the man suggest the woman phone?

  A. The woman’s friend.               B. The manager of the shop.

  C. The manager of the restaurant.

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第一节(共5小题,每小题1.5分,满分7.5分)

听下面5段对话,每段对话后有一个小题,从题中所给的A、B、C三个选项中选出最佳选项,并标在试卷的相应位置。每段对话仅读一遍。

1. When will the woman discuss her class project with the man?

A. During the man’s class.    B. After today’s class.   C. Tomorrow.

2. Where does the conversation most probably take place?

A. In a garden.        B. In a kitchen.     C. In a market.

3. What will the woman do tomorrow morning?

A. See Lisa off.        B. Deal with an e-mail .  C. Go to the zoo.

4. What does the woman suggest the man do?

A. Eat something good for breakfast.          B. Go to work by car.

  C. Exercise just after getting up.

5. What size does the man want?

  A. Size 9.          B. Size 35.       C. Size 39.

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44. 1) 实验原理:不同氨基酸在扩展剂的溶解度不同,所以在滤纸上的扩散速度不同,从而出现在滤纸的不同位置上。

2)实验步骤:

①  6  ② 用毛细管吸取氨基酸样品点在标记上 ,干燥后重复2-3次 。

③ 点样的一端在下,扩展剂的液面要低于点样线

①   用喷雾器向滤纸上均匀喷上0.1%的茚三酮溶液,待干燥后,观察并记录层析斑点的位置.

3)略

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43.  (1)乙,  甲.  (2)a处,   磷酸二酯键

(3) 绿色硬纸板(DNA分子甲)的碱基序列为:(2分)

 ……ATAGCATGCTATCCATG|| AATTCGGCATAC……

  ……TATCGTACGATAGGTACTTAA||GCCGTATG……

红色硬纸纸(DNA分子乙)的碱基序列为:

  ……TCCTAG||AATTCTCGGTATG||AATTCCATAC……

  ……AGGATCTTAA||GAGCCATACTTAA||GGTATG……

  (4)受体菌   基因组    部分基因

  (5) ①目的基因:用酶2和4切割,质粒用酶1和2切割,由于1和4切出的是平末端,可以相连,因而目的基因可以与质粒重组。

②一个  不能

③2个  1个

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42. (1)竞争  ,     (2)C

(3)A.方法步骤:

第二步:在甲培养基上接种R细菌,培养一段时间后,除去R细菌,乙培养基上不接种R细菌,作为对照。

第三步:在这两个培养基上分别接种相同的其他细菌,在适宜且相同条件下,培养一段时间。

第四步:观察并记录甲,乙2组细菌的生长情况

B.结果结论:

预期结果: 甲培养基上细菌的生长受到抑制不能繁殖(或长势比乙差);乙培养基上细菌能正常生长繁殖(或长势比甲好)。

结论:  R细菌产生了不利于其他细菌生长和繁殖的物质。

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41.(1)醋酸菌发酵产生醋酸 (酒精被氧化产生醋酸)   (2)果胶酶、蛋白酶

   (3)①海藻酸钠浓度太低,(或将海藻酸钠融化时未等冷却就将活化的酵母菌液倒入)导致固定的酵母菌数量太少

    ②所用菌种产生酒精的酶活性偏低,导致酒精产量偏低或发酵时间延长。

   (4)①对照原则、单一变量原则(缺一不得分)

  ②因为淀粉分子太大难以通过琼脂扩散与淀粉酶接触而导致反应无法进行

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2.显色剂:0.1%水和茚三酮溶液

若该同学想证明混合液确实含有上述5种氨基酸,请利用题给条件设计试验:

1)实验原理:

A.除脯氨酸,羟脯氨酸和茚三酮反应产生黄色物质外,所用a-氨基酸和一切蛋白质都能和茚三酮反应生成蓝紫色物质。

B.在一定条件下,                               

                                 。

2)实验步骤:

①取层析滤纸一张,剪成20厘米长,14厘米宽的滤纸条,在纸的一端距边缘2-3厘米处用铅笔画一条直线,再此直线上每间隔2厘米作一记号,共需作    处记号.

                                      

                          

③将处理好的滤纸条卷成筒状用U形扣固定,放入加好了扩展剂的层析缸中,待扩展剂上升到一定阶段时取出滤纸,自然风干。本步骤的注意事项是:           

                           

                                      

                                .

3)请在下图中绘出滤纸条上的预期结果:(已知扩散速度为赖氨酸<脯氨酸<缬氨酸<苯丙氨酸<亮氨酸)

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1.扩展剂:4份水饱和的正丁醇和1份醋酸的混合物

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