27. Nowadays more young people have suffered from diabetes __________ it is generally realized.

A. than　　　　 B. that　　　　　 C. which　　　　　　 D. what

26. The man didn’t know _________ the thief broke into the house and stole all his valuable things

A. it was when that　 　B. when it was that　　 C. that it was when　　　 D. was it when that

25. There ________ no water for 2 days, the travellers were all thirsty.

A. having been B. had been　　　 C. is　　　　　　　 D. was

24. Her father told her to be careful when _____ the street.

A. he is crossing　 B. she is crossing C. crossed　　 D. crossing

23. Hardly ________ home when he was asked to go back to work.

A. had he arrived　　 B. he had arrived　　 C. did he arrive　 D. he arrived

22. – My key is gone. Have you seen it anywhere?

– Oh, it is in the room _______ we had a talk last night

A. that　 B. which　　　　　　 C. where　 D. what

第一节　　　 单项填空(共15小题；每小题1分，满分15分)

从A、B、C、D四个选项中，选出可以填入空白处的最佳选项，并在答题卡上将该项涂黑。

21. She was admitted to so excellent ________ university that her parents were proud of her.

A. /　　 B. a　 C. an　 D. the

46．(16分)

(1)①不合理(1分)　　 没有设置重复组，结果不具有说服力(2分)

②不合理(1分)　　 1个计数结果与另2个相差太远(或在操作过程中可能出现错误，或只简单地计算了平均值)(2分)

③在设计实验时，一定要涂布至少3个平板作为重复组，才能增强实验的说服力与准确性。在分析实验结果时，一定要考虑所设置的重复组的结果是否相当，结果差距太大，意味着操作有误，需要重新实验(2分)

④固体培养基　 (2分)

⑤用高温蒸汽灭菌(2分)

(2)在(70-80℃)的高温条件下培养混合培养液(2分)

热稳定(耐高温)的DNA聚合酶(2分)