25. There ________ no water for 2 days, the travellers were all thirsty.

A. having been B. had been　　　 C. is　　　　　　　 D. was

24. Her father told her to be careful when _____ the street.

A. he is crossing　 B. she is crossing C. crossed　　 D. crossing

23. Hardly ________ home when he was asked to go back to work.

A. had he arrived　　 B. he had arrived　　 C. did he arrive　 D. he arrived

22. – My key is gone. Have you seen it anywhere?

– Oh, it is in the room _______ we had a talk last night

A. that　 B. which　　　　　　 C. where　 D. what

第一节　　　 单项填空(共15小题；每小题1分，满分15分)

从A、B、C、D四个选项中，选出可以填入空白处的最佳选项，并在答题卡上将该项涂黑。

21. She was admitted to so excellent ________ university that her parents were proud of her.

A. /　　 B. a　 C. an　 D. the

46．(16分)

(1)①不合理(1分)　　 没有设置重复组，结果不具有说服力(2分)

②不合理(1分)　　 1个计数结果与另2个相差太远(或在操作过程中可能出现错误，或只简单地计算了平均值)(2分)

③在设计实验时，一定要涂布至少3个平板作为重复组，才能增强实验的说服力与准确性。在分析实验结果时，一定要考虑所设置的重复组的结果是否相当，结果差距太大，意味着操作有误，需要重新实验(2分)

④固体培养基　 (2分)

⑤用高温蒸汽灭菌(2分)

(2)在(70-80℃)的高温条件下培养混合培养液(2分)

热稳定(耐高温)的DNA聚合酶(2分)

45．(每空2分，共10分)

(1)外植体　　 (2)去分化(脱分化)

(3)①未分化细胞群分化形成芽　 　② 细胞分裂素

(4)组织培养形成试管苗的过程属于无性生殖，后代不发生性状分离

44．(10分)

(1)① 防止高温杀死酵母菌　　　 ②　海藻酸钠溶液的浓度偏低，固定的酵母细胞数目较少　 ③　酶分子很小，容易从包埋材料中漏出　　 ④　 能多次重复利用　 降低生产成本(或易与产物分离)

(2)①　 附着在葡萄皮上的野生型酵母菌　　

②　 兼性厌氧型　　　　　　 C₆H₁₂O₆ 　　　2C₂H₅OH+2CO₂+能量　　　　　　　　

③　 直接在果汁中加入人工培养的酵母菌　　　 ④ 重铬酸钾