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32.It is imagination   makes the world colorful, full of vigor and vitality.

    A.where        B.what         C.that          D.when

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31.If you talk nice and polite, people listen to you. If you shout, this is no good,   ?

    A.do you        B.don’t you     C.is it           D.isn’t it

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30.John had to have his car repaired in a garage because it   seriously.

    A.damaged B.was being damaged C.had damaged D.had been damaged

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29.The unemployment rate in this district   from 6% to 5% in the past two years.

    A.has fallen     B.had fallen     C.is falling       D.was falling

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28.Nowadays the roles of husband and wife are not as   defined as before, especially when both partners work and earn money for the family.

    A.clear         B.clearer        C.clearly        D.more clearly

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27.The boss has given everyone a special holiday, so we   go to work tomorrow.

    A.can’t         B.mustn’t        C.needn’t      D.shouldn’t

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26.Treat   to a glass of wine to help you relax at the end of the day.

    A.one          B.oneself        C.you          D.yourself

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Directions: Beneath each of the following sentences there are four choices marked A, B, C and D.Choose the one answer ~at best completes the sentence.

25.Dolly wants to cycle round the world and she is really keen   the idea.

    A.on           B.for            C.at            D.with

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Part A Short Conversations

Directions: In Part A, you will hear ten short conversations between two speakers. At the end of each conversation, a question will be asked about what was said.The conversations and the questions will be spoken only once.  After you hear a conversation and the question about it, read the four possible answers on your paper, and decide which one is the best answer to the question you have heard,

1.A.Japan.       B.Italy.        C.Australia.     D.Egypt.

2.A.Four times.     B.Five times.     C.Ten times.     D.Fourteen times.

3.A.A journalist.     B.A novelist.     C.A sportsman.   D.A newsagent.

4.A.To tell him they are busy.         B.To cancel an appointment.

    C.To invite him to see a film.       D.To ask him to get film tickets.

5.A.He didn’t understand the lecture. B.Professor Smith misused a word.

    C.He couldn’t find Professor Smith’s class. D.Professor Smith kept the class late.

6.A.She should lose five pounds.      B.She can’t gain any more weight.

    C.She should buy some new clothes.               D.She needn’t worry about her weight.

7.A.They’re not in the same direction.  B.He’ll join the woman.

    C.He’s going to Work for IBM.      D.He’s going to buy a computer.

8.A.She learned a lot about characters.               B.She forgot the title of the book.

    C.She also found the book difficult. D.She also read a book about names.

9.A.He’ll be in both places.          B.He has to change his schedule at once.

    C.He’ll go to the party immediately.                D.He regrets that he can’t go to the party.

10.A.He plans to buy a new MP3.     B.He doesn’t need his MP3 until Friday.

    C.He can’t wait until Friday for his MP3.             D.He is going to borrow an MP3.

Part B Passages

Directions: In Part B, you will hear two short passages, and you will be asked three questions on each of the passages.  The passages will be read twice, but the questions will be spoken only once.  When you hear a question, read the four possible answers on your paper and decide which one would be the best answer to the question you have heard.

Questions 11 through 13 are based on the following passage.

11.A.Relationship with parents.       B.Life in the university.

    C.Experience of sharing fiats.      D.Friendship with foreigners.

12.A.The parties were not very enjoyable.              B.The house was too noisy.

    C.They had no time to clean the bathroom.         D.She couldn’t afford the phone bill.

13.A.She doesn’t need to cook every day.             B.She has someone to share feelings.

    C.She can live in a new and quiet place.           D.She can get up as late as she likes.

Questions 14 through 16 are based on the following news.

14.A.What they take along          B.How they travel safely.

    C.Which travel guide they choose  D.How t-hey survive overseas.

15.A.Something mysterious.          B.A strange art form.

    C.Something he is good at.        D.Part of every life.

16.A.Doing experiments.             B.Helping athletes compete in games.

    C.Checking the alcohol level      D.Testing the safety of food.

Part C Longer Conversations

Directions: In Part C, you will hear two longer conversations. The conversations will be read twice.  After you hear each conversation, you are required to fill in the numbered blanks with the information you have heard.Write your answers on your answer sheet.

Blanks 17 through 20 are based on the following conversation.

Market Research Interview
Interviewee:        Jack
Length of Net Experience:              About  17  months
Monthly Expense:     £10 to the  18   provider
Purpose:             Mainly to communicate with people belonging to the same 19 group
Advantage:          Easy to 20 ideas and information with others

Complete the form.  Write ONE WORD for each answer.

Blanks 21 through 24 are based on the following conversation.

When is Mike’s project supposed to be finished?  
    21 
What is Mike’s project about?
The  22  in Canadian cities.
How is Mike going to deal with the book he’s found?
To   23   some pages and charts.
What is the most important requirement in doing the project?
Students should write the project   24  .

Complete the form.  Write NO MORE THAN THREE WORDS for each answer.

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45.(13分)某种野生植物有紫花和白花两种表现型,已知紫花形成的生物化学途径是:

A和a、B和b是分别位于两对染色体上的等位基因,A对a、B对b为显性。

基因型不同的两白花植株杂交,F1紫花:白花=1:1.若将F1紫花植株自交,所得F2植株中紫花:白花=9:7,请回答:

  (1)从紫花形成的途径可知,紫花性状是由_____________对基因控制。

  (2)根据F1紫花植株自交的结果,可以推测F1紫花植株的基因型是      

  (3)紫花形成的生物化学途径中,若中间产物是红色(形成红花),那么基因型为AaBb的植株自交,子一代植株的表现型及比例为:      

  (4)紫花中的紫色物质是一种天然的优质色素,但由于B基因表达的酶较少,紫色物质含量较低。设想通过基因工程技术,采用重组的Ti质粒转移一段DNA进入细胞并且整合到染色体上,以促进B基因在花瓣细胞中的表达,提高紫色物质含量。该过程要用到的工具酶有:_____________,Ti质粒的作用是      

  (5)限制性内切酶I的识别序列和切点是-GGATCC一,限制性内切酶II的识别序列和切点是一GATC一。在质粒上有酶I的一个切点,在目的基因的两侧各有1个酶II的切点。

①请画出质粒被限制酶I切割后所形成的黏性末端。

②请画出目的基因两侧被限制酶II切割后所形成的黏性末端。

③在DNA连接酶的作用下,上述两种不同限制酶切割后形成的黏性末端能否连接起来?为什么?

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